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Related Concept Videos

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Related Experiment Video

Updated: Jun 21, 2025

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays
08:37

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

Published on: April 18, 2025

313

Chirality-Dependent Reprogramming of Macrophages.

Jing Han1, Huihui Liu1, Junyu Chen2

  • 1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences; Beijing 100190, China.

ACS Nano
|July 16, 2024
PubMed
Summary
This summary is machine-generated.

Chiral gold particles show distinct effects on macrophage activation. D-Au particles induce an inflammatory metabolic shunt via creatine kinase muscle-type (CKM), impacting cytokine release and offering potential for chiral anticancer therapies.

Keywords:
chiral gold particlescreatinecreatine kinase muscle-typemacrophagesmetabolic reprogramming

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Area of Science:

  • Biomaterials Science
  • Cellular Metabolism
  • Immunology

Background:

  • Chirality of materials influences biological interactions.
  • Cellular metabolic reprogramming by chiral materials is not fully understood.

Purpose of the Study:

  • Investigate the impact of chiral gold nanoparticles on macrophage metabolic reprogramming.
  • Elucidate the mechanisms underlying chiral material-induced macrophage activation.

Main Methods:

  • Synthesis of chiral gold particles using a light-driven approach.
  • Assessment of macrophage activation and metabolic changes.
  • Pharmacological and genetic inhibition of creatine kinase muscle-type (CKM).

Main Results:

  • D-Au particles showed enhanced macrophage activation compared to l-Au particles.
  • D-Au stimulation induced an inflammatory creatine-phosphocreatine shunt via CKM upregulation.
  • CKM inhibition reduced pro-inflammatory cytokine secretion (CXCL2, IL-1β) and NF-κB/NLRP3 inflammasome activation.

Conclusions:

  • Chiral gold nanoparticles can reprogram macrophage metabolism.
  • CKM plays a key role in mediating d-Au induced inflammatory responses.
  • Findings are significant for developing chiral-based anticancer therapies.