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Related Concept Videos

Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

562
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
562

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Related Experiment Video

Updated: Jun 20, 2025

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
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Human B Cell Receptor Repertoire Sequencing.

Prasanti Kotagiri1, Rachael J M Bashford-Rogers2,3, Vanessa L Bryant4,5,6

  • 1Immunology Alfred Hospital, Central Clinical School, Monash University, Melbourne, VIC, Australia. prasanti.kotagiri@monash.edu.

Methods in Molecular Biology (Clifton, N.J.)
|July 17, 2024
PubMed
Summary
This summary is machine-generated.

High-throughput sequencing reveals B cell receptor (BCR) complexity but faces bias. This study optimizes BCR library preparation for accurate, reproducible analysis of B cell immunity in health and disease.

Keywords:
B cell memoryB cell receptorBCR repertoireCell sortingHuman B cellsNext generation sequencing

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Area of Science:

  • Immunology
  • Genomics
  • Molecular Biology

Background:

  • Next-generation sequencing (NGS) offers insights into B cell receptor (BCR) repertoires.
  • Existing NGS methods for BCR repertoire analysis suffer from quantitative bias and sequencing errors.
  • These limitations compromise the accuracy of immune profiling.

Purpose of the Study:

  • To develop an optimized protocol for human BCR repertoire library preparation.
  • To minimize bias and enhance accuracy in high-throughput sequencing of BCR repertoires.
  • To enable reproducible analysis of B cell immunity.

Main Methods:

  • Development of a novel library preparation protocol for human BCRs.
  • Implementation of quality control steps to mitigate bias.
  • Validation of the protocol using high-throughput sequencing.

Main Results:

  • The optimized protocol significantly reduces quantitative bias in BCR repertoire data.
  • Sequencing error rates are minimized, improving data reliability.
  • Reproducible and accurate human BCR repertoire profiles are generated.

Conclusions:

  • The optimized protocol provides a robust method for studying B cell immunity.
  • Accurate BCR repertoire analysis is crucial for understanding health and disease states.
  • This method facilitates advancements in immunological research and diagnostics.