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Updated: Jun 19, 2025
05:05
A Modified Precipitation Method to Isolate Urinary Exosomes
Published on: January 16, 2015
17.6K
Urine beyond electrolytes: diagnosis through extracellular vesicles.
Miriam Anfaiha-Sanchez1, Nerea Lago-Baameiro2, Aranzazu Santiago-Hernandez1
1Departamento de Inmunología, IIS-Fundación Jiménez Díaz -UAM, Madrid, Spain.
Nefrologia
|July 25, 2024
Summary
Extracellular vesicles (EV) in urine can now be directly analyzed using SP-IRIS technology. This non-invasive method quantifies urinary EV and renal proteins, aiding in diagnostics.
Area of Science:
- Biochemistry
- Nanotechnology
- Urology
Background:
- Extracellular vesicles (EV) in urine offer non-invasive insights into kidney health.
- Current EV analysis requires isolation, hindering clinical application.
- Direct analysis of urinary EV is needed for faster diagnostics.
Purpose of the Study:
- To demonstrate the applicability of SP-IRIS technology (ExoView®) for direct urine EV analysis.
- To quantify urinary EV and renal proteins (AMN, SFRP1) using minimal urine volume.
- To validate the potential of this method for clinical diagnostics.
Main Methods:
- Utilized the ExoView® platform with SP-IRIS technology for direct urine EV analysis.
- Quantified EV size, subpopulations (CD63, CD81, CD9), and protein content (AMN, SFRP1).
- Confirmed tubular expression of proteins via immunohistochemistry.
Main Results:
- Direct analysis of EV from 5 μl urine was achieved.
- Identified CD63 as the predominant EV subpopulation (48.92%).
- Quantified AMN and SFRP1 proteins, primarily on CD63+ EV.
Conclusions:
- ExoView® enables direct, rapid analysis of urine EV and associated proteins.
- Minimal sample volume and short analysis time facilitate clinical integration.
- This technology provides a promising non-invasive tool for renal diagnostics.
