Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

4.7K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.7K
Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

4.0K
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Study of electroreflectance spectrum and Franz-Keldysh effect at metal-GaAs interfaces].

Guang pu xue yu guang pu fen xi = Guang pu·2008
Same author

[Study on electro-degradation of new conjugated polymer PFO-BT15 light emitting diodes].

Guang pu xue yu guang pu fen xi = Guang pu·2008
Same author

Comparison of the curative effects of video assisted thoracoscopic anterior correction and small incision, thoracotomic anterior correction for idiopathic thoracic scoliosis.

Chinese medical journal·2008
Same author

Distribution and sources of mercury in soils from former industrialized urban areas of Beijing, China.

Environmental monitoring and assessment·2008
Same author

[Main flavonoids from Sophora flavescenes].

Yao xue xue bao = Acta pharmaceutica Sinica·2008
Same author

External validation and prediction employing the predictive squared correlation coefficient test set activity mean vs training set activity mean.

Journal of chemical information and modeling·2008

Related Experiment Video

Updated: Jun 19, 2025

Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells
14:04

Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells

Published on: August 1, 2020

5.9K

Tissue-engineered mesenchymal stem cell constructs alleviate tendinopathy by suppressing vascularization.

Dijun Li1,2,3, Jingwei Jiu1,2, Haifeng Liu1,2

  • 1Department of Orthopaedic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, China.

Bioactive Materials
|July 26, 2024
PubMed
Summary

Tissue engineered stem cells (TSCs) limit pathological vascularization in tendinopathy by inhibiting VEGF receptor activation. This novel approach reduces inflammation and promotes tendon healing, offering a promising therapy for early-stage tendinopathy.

More Related Videos

Evaluation of Stem Cell Therapies in a Bilateral Patellar Tendon Injury Model in Rats
09:31

Evaluation of Stem Cell Therapies in a Bilateral Patellar Tendon Injury Model in Rats

Published on: March 30, 2018

11.2K
Treatment of Osteochondral Defects in the Rabbit's Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots
11:22

Treatment of Osteochondral Defects in the Rabbit's Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots

Published on: May 21, 2013

17.4K

Related Experiment Videos

Last Updated: Jun 19, 2025

Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells
14:04

Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells

Published on: August 1, 2020

5.9K
Evaluation of Stem Cell Therapies in a Bilateral Patellar Tendon Injury Model in Rats
09:31

Evaluation of Stem Cell Therapies in a Bilateral Patellar Tendon Injury Model in Rats

Published on: March 30, 2018

11.2K
Treatment of Osteochondral Defects in the Rabbit's Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots
11:22

Treatment of Osteochondral Defects in the Rabbit's Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots

Published on: May 21, 2013

17.4K

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Orthopedics

Background:

  • Tendinopathy involves chronic inflammation and damage driven by imbalanced angiogenesis.
  • Current stem cell therapies and scaffolds show limited efficacy in treating tendinopathy.
  • Targeting early pathological vascularization presents a potential therapeutic strategy for tendon regeneration.

Purpose of the Study:

  • To investigate the efficacy of tissue engineered stem cells (TSCs) combined with microcarrier scaffolds in managing tendinopathy.
  • To evaluate the potential of TSCs to limit excessive vascularization and modulate the tenocyte inflammatory and anabolic profile.

Main Methods:

  • Human umbilical cord mesenchymal stem cells (hUC-MSCs) were used to generate TSCs.
  • TSCs were combined with microcarrier scaffolds to limit vascularization in a tendinopathy model.
  • In vitro assays assessed angiogenesis and vascular endothelial cell proliferation.
  • In vivo studies involved local injection of TSCs into rats with collagenase-induced tendinopathy.
  • Transcriptome sequencing was employed to elucidate the underlying mechanisms.

Main Results:

  • TSCs reduced in vitro angiogenesis and vascular endothelial cell proliferation by inhibiting VEGF receptor activation.
  • TSCs decreased tenocyte inflammatory expression while enhancing anabolic and tenogenic characteristics.
  • Local TSC injection in rats significantly reduced inflammation and vascularization in early-stage tendinopathy.
  • Transcriptome analysis identified Rap1-mediated vascular inhibition as a key mechanism.

Conclusions:

  • TSCs represent a novel therapeutic approach for suppressing pathological tendon vascularization.
  • This strategy shows promise for treating early-stage tendinopathy by modulating angiogenesis and inflammation.
  • The Rap1-mediated pathway offers a mechanistic insight into TSC efficacy.