Luminescence Probes in Bio-Applications: From Principle to Practice

Area of Science:

• Biomedical Optics
• Analytical Chemistry
• Molecular Imaging

Background:

• Optical imaging-based bioanalysis has advanced significantly.
• Luminescence probes are crucial for detecting and tracing biomolecules in biological systems.
• Understanding biomolecule roles requires advanced detection methods.

Purpose of the Study:

• To review the working principles of various luminescence probes.
• To summarize recent advances in bio-applications of luminescence probes.
• To provide a comprehensive understanding of modern luminescence probe technologies.

Main Methods:

• Review of current literature on luminescence probes.
• Categorization of luminescence probes based on emission type.
• Summarization of bio-applications by detection and therapeutic use.

Main Results:

• Detailed description of fluorescence, bioluminescence, chemiluminescence, afterglow, photoacoustic, and Cerenkov luminescence probes.
• Overview of applications including metal ion detection, secretion monitoring, in vivo imaging, and therapeutic interventions.
• Highlighting the versatility and progress in luminescence probe development.

Conclusions:

• Luminescence probes are essential tools in modern bioanalysis and molecular imaging.
• Continued development promises enhanced sensitivity and specificity for biological studies.
• These probes offer significant potential for diagnostics and targeted therapies.