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Development and Assessment of Multiple Illumination Color Fourier Ptychographic Microscopy for High Throughput Sample
Patrik Gilley1, Ke Zhang2, Neman Abdoli1
1School of Electrical and Computer Engineering, University of Oklahoma, Norman, OK 73019, USA.
Sensors (Basel, Switzerland)
|July 27, 2024
Summary
This study introduces multiplexed color illumination for Fourier ptychography microscopy (FPM), enhancing data acquisition efficiency. This novel approach achieves comparable imaging performance to conventional methods, paving the way for high-throughput FPM systems.
Area of Science:
- Microscopy and Imaging Technologies
- Optical Engineering
- Biomedical Imaging
Background:
- Fourier ptychography microscopy (FPM) offers high-resolution imaging but can be limited by data acquisition speed.
- Conventional FPM typically uses sequential single-channel LED illumination, which is time-consuming.
Purpose of the Study:
- To develop and validate a multiplexed color illumination strategy to improve FPM data acquisition efficiency.
- To assess the imaging performance and clinical utility of the proposed multiplexed illumination method.
Main Methods:
- A multiplexed color illumination strategy was proposed, utilizing multiple white light LEDs simultaneously for each image acquisition with a color camera.
- An FPM prototype with a 4×/0.13 NA objective lens was developed, achieving a 20×/0.4 NA equivalent resolution.
- Two- and four-LED illumination patterns were experimentally applied, and imaging performance was evaluated using a USAF 1951 resolution target, H&E tissue samples, and metaphase chromosome cells.
Main Results:
- Multiplexed illumination (two- or four-LED) demonstrated comparable imaging performance across all three MTF curve channels to single-LED illumination.
- Reconstructed images of tissue and cells successfully preserved the detailed structures of nuclei and cytoplasm.
- The multiplexed method showed improved preservation of cell edges compared to conventional microscopes.
Conclusions:
- Multiplexed color illumination is a feasible strategy for enhancing FPM data acquisition efficiency.
- This approach maintains high imaging fidelity and shows potential for developing high-throughput FPM scanning systems.
