Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

2.3K
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
2.3K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

7.3K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.3K
The Extracellular Matrix01:29

The Extracellular Matrix

8.9K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
8.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advanced biomaterial strategies for 3D <i>in vitro</i> biomimetic neuroblastoma cell culture.

Biomaterials science·2026
Same author

Proteomic composition and mutual assembly of the C2a projection in vertebrate motile cilia.

eLife·2026
Same author

Robust Quantum Cutting via Halide-Bearing Ligand Passivation and Gradient Halide Reconstruction for Ultrabroadband Ultraviolet-to-Near-Infrared Photodetection and Imaging.

ACS nano·2026
Same author

NiO<sub><i>x</i></sub>/SAM-Mediated Interface Engineering for High-Performance PEA<sub>2</sub>SnI<sub>4</sub> Pure-Red Perovskite Light-Emitting Diodes.

The journal of physical chemistry letters·2026
Same author

RGS22 is a metazoa-specific radial spoke component required for coordinated ciliary beating.

Nature communications·2026
Same author

The predictive value of nurse-led preoperative frailty assessment for postoperative delirium in liver transplant recipients: a retrospective cohort study.

BMC anesthesiology·2026

Related Experiment Video

Updated: Jun 18, 2025

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach
10:23

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Published on: August 26, 2013

13.9K

BMP-Binding Polysulfonate Brushes to Control Growth Factor Presentation and Regulate Matrix Remodelling.

Metzli Hernandez Marchena1, Elisa Lambert2, Bojana Bogdanović2

  • 1School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London E1 4NS, U.K.

ACS Applied Materials & Interfaces
|July 29, 2024
PubMed
Summary

Biomimetic poly(sulfopropyl methacrylate) (PSPMA) brushes offer stable capture of bone morphogenetic proteins (BMPs) for tissue repair. These GAG-mimetic interfaces enhance BMP2 binding and matrix deposition, advancing regenerative medicine scaffolds.

Keywords:
ATRPBMP2biomimeticpolymer brushsulfonate

More Related Videos

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery
12:27

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Published on: August 22, 2016

7.6K
Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
11:20

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

7.6K

Related Experiment Videos

Last Updated: Jun 18, 2025

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach
10:23

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Published on: August 26, 2013

13.9K
Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery
12:27

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Published on: August 22, 2016

7.6K
Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
11:20

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

7.6K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Surface Chemistry

Background:

  • Bone morphogenetic proteins (BMPs) are crucial for tissue repair but require stable immobilization on biomaterials.
  • Heparin, a glycosaminoglycan (GAG), captures BMPs but has poor stability and material compatibility.
  • Developing stable, biomimetic interfaces for BMPs is essential for advanced regenerative medicine.

Purpose of the Study:

  • To develop and characterize poly(sulfopropyl methacrylate) (PSPMA) brushes as stable, biomimetic GAG interfaces for BMP capture.
  • To investigate the binding capacity and stability of BMP2 and BMP4 on PSPMA brushes.
  • To evaluate the impact of BMP2-functionalized PSPMA brushes on cell behavior and matrix deposition.

Main Methods:

  • Surface-initiated activator regenerated by electron transfer polymerization for PSPMA brush growth.
  • Ellipsometry, X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR) for characterization.
  • Surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring (QCM-D) for BMP binding analysis.
  • Cell culture studies with dermal fibroblasts to assess matrix deposition.

Main Results:

  • PSPMA brushes were successfully synthesized and characterized for swelling and surface chemistry.
  • PSPMA brushes demonstrated stable and high-density binding of BMP2 and BMP4, with brush architecture significantly impacting capacity.
  • BMP2 showed primary adsorption on thick, dense brushes, ensuring receptor accessibility.
  • BMP2-functionalized PSPMA brushes significantly enhanced matrix deposition in fibroblast cultures.

Conclusions:

  • PSPMA brushes serve as effective biomimetic GAG interfaces for stable BMP capture.
  • These brushes offer a promising platform for scaffold engineering in regenerative medicine.
  • Stable BMP2 immobilization via PSPMA brushes can positively influence cellular responses and tissue regeneration.