Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unilateral extrapedicular vs. bilateral transpedicular percutaneous kyphoplasty in osteoporotic vertebral compression fractures: an exploratory systematic review and meta-analysis.

Frontiers in surgery·2026
Same author

Transverse process-pedicle versus conventional transpedicular approach in percutaneous kyphoplasty for osteoporotic vertebral compression fractures: a systematic review and pilot meta-analysis.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2026
Same author

On the Impact of Chronic Message Fatigue on Informational and Behavioral Outcomes.

Health communication·2026
Same author

Efficacy of acupuncture in managing acute exacerbation of chronic obstructive pulmonary disease: a network meta-analysis.

Frontiers in medicine·2026
Same author

Theoretical Investigation on Lithographic Properties of Zinc-Oxo and Cadmium-Oxo Clusters Ligated with Differently Substituted Benzoic Acids.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Trends in Weight Loss Attempts and Strategies Among US Adolescents With Overweight or Obesity.

Obesity (Silver Spring, Md.)·2026

Related Experiment Video

Updated: Jun 18, 2025

Comparative Lesions Analysis Through a Targeted Sequencing Approach
08:16

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

6.7K

A transcriptomic analysis of incisional hernia based on high-throughput sequencing technology.

Yiming Lin1, Hekai Shi1, Rongduo Yang1

  • 1Department of General Surgery, Fudan University Affiliated Huadong Hosptial, No.221, West Yan'an Road, Jing'an District, Shanghai, 200040, Republic of China.

Hernia : the Journal of Hernias and Abdominal Wall Surgery
|July 29, 2024
PubMed
Summary

This study used high-throughput sequencing to identify key genes and pathways involved in incisional hernias. Findings reveal new targets for preventing and treating this common postoperative complication.

Keywords:
Extracellular matrixIncisional herniaSuperficial fasciaTranscriptomics

More Related Videos

Application of a New Mesh Fixation Method in Laparoscopic Incisional Hernia Repair
05:15

Application of a New Mesh Fixation Method in Laparoscopic Incisional Hernia Repair

Published on: December 23, 2022

6.2K
gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
08:15

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Published on: October 6, 2014

12.2K

Related Experiment Videos

Last Updated: Jun 18, 2025

Comparative Lesions Analysis Through a Targeted Sequencing Approach
08:16

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

6.7K
Application of a New Mesh Fixation Method in Laparoscopic Incisional Hernia Repair
05:15

Application of a New Mesh Fixation Method in Laparoscopic Incisional Hernia Repair

Published on: December 23, 2022

6.2K
gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
08:15

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Published on: October 6, 2014

12.2K

Area of Science:

  • * Molecular biology
  • * Genomics
  • * Surgical pathology

Background:

  • * Incisional hernia is a frequent surgical complication.
  • * Limited transcriptomic data exists for incisional hernias.
  • * Understanding molecular mechanisms is crucial for treatment.

Purpose of the Study:

  • * To explore the pathogenesis of incisional hernias using transcriptomic analysis.
  • * To identify potential therapeutic targets for incisional hernias.
  • * To investigate gene expression profiles in fascial tissue.

Main Methods:

  • * Second-generation high-throughput sequencing of superficial fasciae from hernia patients and controls.
  • * Analysis of differentially expressed genes (DEGs) and gene ontology.
  • * Utilization of an external dataset to validate key DEGs.

Main Results:

  • * Identified 1,823 DEGs linked to extracellular matrix imbalance, inflammation, and collagen changes.
  • * Pinpointed eight key DEGs (TNNT3, CMAY5, ATP1B4, ASB5, CILP, SIX4, FBN1, FNDC5).
  • * Revealed non-alcoholic fatty liver disease, TNF, and IL-17 signaling pathways as significant.

Conclusions:

  • * Identified eight key DEGs and three pathways associated with incisional hernias.
  • * Provided novel insights into incisional hernia pathogenesis.
  • * Highlighted potential targets for hernia prevention and treatment.