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Related Concept Videos

Teeth01:15

Teeth

369
The formation of teeth, also known as odontogenesis, is a complex process that begins in utero, around the sixth week of embryonic development. There are three stages to this process: the bud stage, the cap stage, and the bell stage.
In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin...
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Determination01:51

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During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
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Related Experiment Video

Updated: Jun 18, 2025

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
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Spatial and temporal gene expression patterns during early human odontogenesis process.

Yejia Yu1, Kun Wang2, Zhuo Wang1

  • 1State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases and Engineering Research Center of Oral Translational Medicine, Ministry of Education and National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Frontiers in Bioengineering and Biotechnology
|July 31, 2024
PubMed
Summary
This summary is machine-generated.

This study details early human tooth development using RNA sequencing and Laser-capture microdissection. Key events like angiogenesis and ECM organization were identified, offering insights into tooth regeneration.

Keywords:
RNA sequencinghuman deciduous tooth germlaser-capture microdissectionodontogenesisspecies comparison

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Area of Science:

  • Developmental Biology
  • Genomics
  • Regenerative Medicine

Background:

  • Odontogenesis, the process of tooth formation, is critical for treating dental abnormalities and tooth loss.
  • Early human odontogenesis remains understudied, limiting our understanding of tooth development and regeneration strategies.

Purpose of the Study:

  • To investigate the spatiotemporal transcriptomic landscape of early human deciduous tooth germs.
  • To identify key molecular events and pathways involved in human odontogenesis.
  • To provide a foundation for future tooth regeneration research.

Main Methods:

  • Combined RNA sequencing (RNA-seq) with Laser-capture microdissection (LCM) for high-resolution transcriptomic analysis.
  • Analyzed human deciduous tooth germs from the cap to early bell stages.
  • Performed species comparisons with mice to identify conserved developmental events.

Main Results:

  • Identified hallmark events during odontogenesis, including angiogenesis, ossification, axonogenesis, and extracellular matrix (ECM) organization.
  • Highlighted the crucial role of ECM in regulating tooth-inductive capabilities.
  • Revealed conserved odontogenic processes between humans and mice.

Conclusions:

  • This study provides a comprehensive transcriptomic map of early human tooth development.
  • The findings offer new perspectives on understanding tooth development and potential strategies for tooth regeneration.
  • Enriches the transcriptomic database for human tooth development at critical early stages.