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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jun 18, 2025

Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation

Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation

Published on: November 3, 2018

Flow Cytometric Assessment of Malignant Hematologic Disorders.

Connor M Hartzell¹, Aaron C Shaver¹, Emily F Mason¹

¹Department of Pathology, Microbiology & Immunology, Vanderbilt University Medical Center, 445 Great Circle Road, Nashville, TN 37228, USA.

Clinics in Laboratory Medicine

|August 1, 2024

Summary

Multiparameter flow cytometry (MPF) aids in diagnosing blood cancers by detecting T-cell issues and myeloid dysplasia. Advanced MPF tools improve minimal residual disease monitoring for better patient management in leukemia and other hematologic diseases.

Keywords:

Clonality Flow cytometry Hematologic malignancy Minimal residual disease TRBC1

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Last Updated: Jun 18, 2025

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Colony Forming Cell CFC Assay for Human Hematopoietic Cells

Colony Forming Cell CFC Assay for Human Hematopoietic Cells

Published on: December 18, 2010

Area of Science:

Hematology
Immunology
Oncology

Background:

Multiparameter flow cytometry (MPF) is crucial for diagnosing hematologic malignancies.
Recent advancements enhance MPF's role in identifying T-cell clonality and myelomonocytic dysplasia.
Minimal/measurable residual disease (MRD) analysis is vital for B-lymphoblastic leukemia and increasingly important for myeloid malignancies.

Purpose of the Study:

To highlight the expanding utility of MPF in hematologic disease diagnosis.
To emphasize the role of emerging data analysis tools in leveraging complex MPF assays.
To underscore the importance of MPF in managing hematologic malignancies.

Main Methods:

Utilizing multiparameter flow cytometry (MPF) for diagnostic workup.
Applying recently developed tools for T-cell clonality and myelomonocytic dysplasia detection.
Implementing MRD analysis in the management of B-lymphoblastic leukemia and myeloid malignancies.

Main Results:

MPF effectively detects T-cell clonality and myelomonocytic dysplasia.
MRD analysis is a critical component in managing B-lymphoblastic leukemia and shows promise in myeloid malignancies.
Increasing complexity of MPF assays necessitates advanced data analysis tools.

Conclusions:

MPF is indispensable for diagnosing hematologic malignancies.
Emerging analytical tools are essential for maximizing the benefits of complex MPF assays.
MPF plays a key role in both diagnosis and disease monitoring of hematologic cancers.