Chitosan-coated manganese ferrite nanoparticles enhanced Rhodotorula toruloides carotenoid production
Nayra Ochoa-Viñals1, Dania Alonso-Estrada1, Rodolfo Ramos-González2
1Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Unidad Saltillo, 25280, Saltillo, Coahuila, Mexico.
Bioprocess and Biosystems Engineering
|August 1, 2024
Summary
Chitosan-coated magnetic nanoparticles (MnFe2O4-CS) enhance carotenoid production in Rhodotorula toruloides yeast. This nanobiotechnology offers a promising method for increased metabolite yield and efficient biomass separation.
Area of Science:
- Biotechnology
- Nanotechnology
- Microbiology
Background:
- Rhodotorula toruloides is a yeast known for producing valuable carotenoids.
- Magnetic nanoparticles offer unique properties for biological applications.
- Optimizing carotenoid production and biomass recovery is crucial for industrial applications.
Purpose of the Study:
- To investigate the interaction between Rhodotorula toruloides and magnetic nanoparticles (MnFe2O4 and MnFe2O4-CS).
- To evaluate the effect of these nanoparticles on carotenoid production in R. toruloides.
- To explore the potential of magnetic nanoparticles for cell immobilization and biomass separation.
Main Methods:
- Synthesis of manganese ferrite (MnFe2O4) and chitosan-coated (MnFe2O4-CS) nanoparticles via co-precipitation and hydrothermal treatment.
- Characterization of nanoparticles using XRD, Magnetometry, DLS, and FTIR.
- Kinetic study of cell immobilization, adsorption isotherm modeling, and cell viability assessment (Trypan blue assay).
Main Results:
- Chitosan coating altered nanoparticle size and magnetic properties.
- Optimal cell immobilization equilibrium time was 2 hours.
- Carotenoid production significantly increased to 256.2 µg/g dry mass with MnFe2O4-CS at 2.0 mg/mL.
- Viability of R. toruloides remained high after interaction with nanoparticles.
Conclusions:
- Chitosan-coated magnetic nanoparticles (MnFe2O4-CS) effectively stimulate carotenoid production in R. toruloides.
- Magnetic separation of yeast biomass is a viable and efficient technique.
- This nanobiotechnological approach presents a promising strategy for enhancing yeast metabolite production and recovery.
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