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Preparation of Viral DNA from Nucleocapsids
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Temperature-dependent ejection evolution arising from active and passive effects in DNA viruses.

Cheng-Yin Zhang1, Neng-Hui Zhang2

  • 1Shanghai Institute of Applied Mathematics and Mechanics, Shanghai Key Laboratory of Mechanics in Energy Engineering, School of Mechanics and Engineering Science, Shanghai University, Shanghai, China; Department of Engineering Mechanics, Faculty of Civil Engineering and Mechanics, Kunming University of Science and Technology, Kunming, China.

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Summary
This summary is machine-generated.

Virus ejection velocity increases with temperature due to altered DNA mechanics and viral portal size. This study models temperature effects on DNA virus ejection dynamics, revealing key mechanisms.

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Area of Science:

  • Biophysics
  • Molecular Biology
  • Computational Biology

Background:

  • DNA virus ejection velocity is temperature-dependent, impacting infection.
  • Quantifying multiscale viral systems is challenging, limiting theoretical studies.
  • Systematic research on temperature-dependent ejection dynamics is lacking.

Purpose of the Study:

  • To develop a multiscale model for exploring temperature-dependent mechanical properties during virus ejection.
  • To quantitatively analyze the underlying mechanisms of temperature-influenced ejection dynamics.
  • To investigate the impact of temperature on DNA virus infection processes.

Main Methods:

  • Developed a multiscale model incorporating temperature-dependent parameters.
  • Utilized two DNA structural models (two-domain and single-domain) for different ejection stages.
  • Introduced temperature effects via Debye length, DNA persistence length, and kinetic energy.

Main Results:

  • Temperature variations alter the DNA structure's energy landscape within the capsid.
  • Changes in DNA structure affect ejection force and friction, increasing velocity at higher temperatures.
  • Model supports the hypothesis that temperature-induced viral portal size changes enhance DNA ejection.

Conclusions:

  • Temperature significantly influences DNA virus ejection velocity through mechanical property modulation.
  • The multiscale model provides quantitative insights into temperature-dependent viral ejection mechanisms.
  • Findings contribute to understanding virus-host interactions and developing antiviral strategies.