Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

317
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
317
Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

719
Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
719
UV–Vis Spectroscopy: Beer–Lambert Law01:09

UV–Vis Spectroscopy: Beer–Lambert Law

2.5K
The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The...
2.5K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

5.7K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
5.7K
Radiation: Applications01:17

Radiation: Applications

1.1K
The average temperature of Earth is the subject of much current discussion. Earth is in radiative contact with both the Sun and dark space; it receives almost all its energy from the radiation of the Sun and reflects some of it into outer space. Dark space is very cold, about 3 K, so Earth radiates energy into it. For instance, heat transfer occurs from soil and grasses, the rate of which can be so rapid that frost can occur on clear summer evenings, even in warm latitudes.
The average...
1.1K
Absorption of Radiation01:05

Absorption of Radiation

716
The rate of heat transfer by emitted radiation is described by the Stefan-Boltzmann law of radiation:
716

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

On-Chip Raman Spectroscopy for Rapid Antimicrobial Susceptibility Testing from Blood Cultures.

Analytical chemistry·2026
Same author

From bulk samples to single cells: measurement strategies in Raman-based antibiotic susceptibility testing.

Analytical and bioanalytical chemistry·2026
Same author

Current trends in machine learning for surface-enhanced Raman spectroscopy.

The Analyst·2026
Same author

Soft-Matter Confinement Modulates Excited-State Dynamics of Ru Photocatalysts for Hydrogen Evolution in Aqueous Media.

ACS materials Au·2026
Same author

Bioanalysis Using Surface-Enhanced Raman Spectroscopy: From the Perspective of Single Molecules.

ACS nano·2026
Same author

Complex-Valued Chemometrics for Analyzing Absorbance or Raman Spectra.

Analytical chemistry·2026

Related Experiment Video

Updated: Jun 18, 2025

Simulation, Fabrication and Characterization of THz Metamaterial Absorbers
13:44

Simulation, Fabrication and Characterization of THz Metamaterial Absorbers

Published on: December 27, 2012

15.3K

Understanding Advanced Attenuated Total Reflection Correction: The Low Absorbance Assumption.

Thomas G Mayerhöfer1,2, Jürgen Popp1,2

  • 1Leibniz Institute of Photonic Technology (IPHT), Jena, Germany.

Applied Spectroscopy
|August 2, 2024
PubMed
Summary
This summary is machine-generated.

We developed a new attenuated total reflection (ATR) correction method that accounts for polarization and incidence angles. While not perfect, it offers insights into spectral correction and may outperform existing methods.

Keywords:
ATRATR correctionAttenuated total reflectioneffective thicknesslow absorption assumption

More Related Videos

Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy
09:25

Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy

Published on: August 22, 2018

12.4K
Author Spotlight: A Stable Phantom Material for Optical and Acoustic Imaging
04:54

Author Spotlight: A Stable Phantom Material for Optical and Acoustic Imaging

Published on: June 16, 2023

2.8K

Related Experiment Videos

Last Updated: Jun 18, 2025

Simulation, Fabrication and Characterization of THz Metamaterial Absorbers
13:44

Simulation, Fabrication and Characterization of THz Metamaterial Absorbers

Published on: December 27, 2012

15.3K
Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy
09:25

Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy

Published on: August 22, 2018

12.4K
Author Spotlight: A Stable Phantom Material for Optical and Acoustic Imaging
04:54

Author Spotlight: A Stable Phantom Material for Optical and Acoustic Imaging

Published on: June 16, 2023

2.8K

Area of Science:

  • Spectroscopy
  • Analytical Chemistry
  • Physical Chemistry

Background:

  • Attenuated total reflection (ATR) spectroscopy is a widely used technique for analyzing various samples.
  • Accurate spectral data is crucial for reliable interpretation in ATR spectroscopy.
  • Existing ATR correction methods have limitations, particularly concerning polarization and angle of incidence.

Purpose of the Study:

  • To present a novel ATR correction scheme.
  • To address limitations in current ATR spectral correction methods.
  • To provide insights into the weak absorption approximation in ATR spectroscopy.

Main Methods:

  • Development of an ATR correction scheme considering polarization state.
  • Application of the scheme for arbitrary angles of incidence above the critical angle.
  • Comparison with proprietary ATR correction methods, such as ThermoFisher's advanced ATR correction.

Main Results:

  • The proposed ATR correction scheme rectifies ATR spectra by considering polarization and arbitrary angles of incidence.
  • The method relies on the weak absorption approximation, thus not achieving perfect correction.
  • The scheme's performance can surpass proprietary methods for specific polarization states and instrument configurations.

Conclusions:

  • The developed ATR correction scheme offers a transparent and potentially superior alternative for spectral rectification.
  • Understanding this method provides valuable insights into the weak absorption approximation.
  • The scheme enhances the accuracy and reliability of ATR spectroscopic analyses.