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Updated: Jun 18, 2025
Detection of Protease Activity by Fluorescent Peptide Zymography
Published on: January 20, 2019
A sensitive benzothiazole fluorescent probe for the detection of γ-glutamyl transpeptidase activity and its
Xiaoming Wu1, Leyuan Ding1, Shaoxiang Yang1
1Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University, Beijing, China.
Abstract:
A sensitive benzothiazole fluorescent probe (PBZO) for the detection of γ-glutamyl transpeptidase (GGT) activity was developed. Based on the enzymatic hydrolysis of peptide bonds by glutamyl transpeptidase, it can be specifically recognized by PBZO. The PBZO has a good linear relationship with different gradients of GGT activity at the emission wavelength of 560 nm, the Stokes shift reached 215 nm, and the detection limit of GGT activity is 0.1644 U/ml. With the increase of GGT concentration in the probe solution, the color of the solution gradually changed from orange to dark yellow under the 365 nm UV lamp. The same color change was also observed on the probe test paper. In addition, there is a linear relationship between the GGT activity and the R-value of the probe solution. More importantly, the probe has a good recovery rate in serum. Therefore, this probe can be used as a convenient tool for detecting GGT activity.
