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An enhanced and rapid method for von Willebrand factor multimer analysis for mechanical circulatory device testing.
Amanda G Smith1, Antony P McNamee1, Chris H H Chan2
1Biorheology Research Laboratory, Menzies Health Institute Queensland, Griffith University, Gold Coast, Queensland, Australia.
Artificial Organs
|August 5, 2024
Summary
This study optimized Von Willebrand factor (VWF) multimer analysis, reducing time from 3 days to 8 hours. The new method improves high molecular weight VWF resolution and offers reproducible results for bleeding disorder diagnosis during mechanical circulatory support use.
Area of Science:
- Biochemistry and Hematology
- Medical Device Technology
Background:
- Von Willebrand factor (VWF) is crucial for hemostasis and diagnosing bleeding disorders.
- Current VWF multimer analysis is inconsistent, costly, and time-consuming, requiring expert analysis.
- There is a need for efficient and reproducible VWF multimer analysis, especially for mechanical circulatory support (MCS) related bleeding.
Purpose of the Study:
- To streamline and optimize VWF multimer analysis for improved efficiency and reproducibility.
- To develop a faster method for VWF multimer analysis.
- To aid in identifying or predicting bleeding disorders induced by MCS.
Main Methods:
- Blood samples from healthy volunteers were subjected to high shear forces using a ventricular assist device.
- VWF multimers were analyzed via vertical-gel agarose electrophoresis and Western blotting.
- Densitometry was used to analyze VWF distribution, comparing proprietary and open-source software.
Main Results:
- Protocol duration was reduced from three days to approximately eight hours.
- Resolution of high molecular weight (HMW) VWF multimers was significantly enhanced.
- Densitometric analysis showed strong correlation between software types, validating the tools.
Conclusions:
- The developed methodology is recommended for affordable, accurate, and reproducible VWF multimer evaluation during MCS use and testing.
- This optimized method can aid in diagnosing bleeding disorders associated with mechanical circulatory support.
- Further research comparing this method with semi-automated approaches is suggested for inter-laboratory consistency.
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