Prepared Sandwich structure WS₂/ag@MIP composite for ultrasensitive SERS detection of trace 17β-estradiol in food

17β-E₂ is used in animal growth regulation and agricultural fertilizer, and even ng L^-1 mass concentration levels can show biological effects. In this work, Ag NPs was used as surface-enhanced Raman spectroscopy (SERS) source and WS₂ was synthesized by a simple method to provide a uniform distribution platform for Ag NPs. The MIP was the shell, which can selectively enrich the target molecule, pull the distance between the target molecule and SERS source, and protect Ag NPs. A cyclable SERS substrate with high sensitivity for detecting 17β-E₂ in food was constructed. The optimized WS₂/Ag@MIP as SERS substrate has the advantages of high Enhanced Factor (EF = 2.78 × 10⁹), low detection limit (LOD = 0. 0958 pM), strong anti-interference ability, and good recycling performance. Moreover, the detection of 17β-E₂ in real samples still has good accuracy. This work provides a new possibility for the trace detection of 17β-E₂ in food.