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Updated: Jun 17, 2025

A Micropatterning Assay for Measuring Cell Chirality
Published on: March 11, 2022
Chirality and Curvature on Protein Adsorption and Osteogenesis
Meijun Li1, Shengjie Jiang2, Xiaowei Wang2
1Beijing National Laboratory for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, No.2, 1st North Street, Zhongguancun, Beijing, 100190, P.R.China.
None:
Here we designed enantiomeric lipid-mimetic glutamic acid derivatives (L/D-UG) and investigated their self-assembled chiral nanostructures' interaction with the protein adsorption as well as the osteogenesis. It was found that L or D-UG molecules can self-assemble into vesicle bilayers and two-dimensional (2D) nanocrystals via a kinetic and thermodynamic control, respectively. These chiral vesicles and 2D crystals showed differentiated adsorption of proteins, determined by their curvature and chirality. Specifically, fibronectin constituted by L-amino acids adsorbed preferentially on L-UG 2D crystal in a semi-random pattern and L-2D nanocrystal show as the most effective structures to promote bone regeneration. The controlled vesicle and 2D crystal assemblies with different chirality and curvature helps to clarify their determine roles in protein adsorption and osteogenesis.
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