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Updated: Jun 17, 2025

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Chromosome-level assembly and evolution analysis of the Trichosanthes truncata genome
Ying Hu1,2, Xiaomei Wei1,2, Zhuannan Chu3
1National Center for Traditional Chinese Medicine Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, 530023, China.
Abstract:
Trichosanthes truncata C. B. Clarke, an important medicinal plant, is a dioecious plant belonging to the Cucurbitaceae family. This study presents a chromosomal-level reference genome assembly for T. truncata. Through the integration of PacBio high-fidelity sequencing and high-throughput chromosome conformation capture technology, a final genome sequence of 637.41 Mb was assembled, with an N50 of 57.24 Mb and consisting of 11 pseudochromosomes. Additionally, 97.21 Mb of repetitive sequences and 36,172 protein-coding genes were annotated. This high-quality genome assembly is of utmost significance for studying the molecular mechanisms underlying the biosynthesis of bioactive compounds. Furthermore, this study provided valuable insights into plant comparative genomics research.
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