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Published on: March 11, 2014
Microfluid biosensor for detection of hpv in patient scraping samples: Determining E6 and E7 oncogenes
Léony S Oliveira1, Sanket Goel2, Khairunnisa Amreen2
1Programa de Pós-Graduação em Inovação Terapêutica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil; Laboratório de Biodispositivos Nanoestruturados, Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
This study developed a novel electrochemical genosensor on a microfluidic chip for detecting high-risk HPV genotypes (HPV16, HPV18) and low-risk genotypes (HPV06, HPV11) in cervical samples, enabling early cancer detection.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Molecular Diagnostics
Background:
- Human Papillomavirus (HPV) E6 and E7 oncogenes are key drivers of cervical cancer.
- Accurate and rapid detection of HPV genotypes is crucial for effective diagnosis and management.
- Current diagnostic methods may lack the sensitivity or specificity for early-stage oncogenic potential detection.
Purpose of the Study:
- To develop and validate a label-free electrochemical genosensor integrated with a microfluidic system.
- To detect and differentiate HPV E6 and E7 oncogenes in clinical cervical scraping samples.
- To assess the sensor's performance in distinguishing between high-risk and low-risk HPV genotypes.
Main Methods:
- Fabrication of a nanostructured sensor using cysteine and graphene quantum dots for enhanced electrochemical properties.
- Integration of the sensor within a microfluidic system for sample processing.
- Voltammetric analysis of cervical scraping samples to measure biorecognition response (ΔI%).
Main Results:
- The genosensor successfully differentiated between low-risk (HPV06, HPV11) and high-risk (HPV16, HPV18) HPV genotypes based on distinct voltammetric responses.
- Biorecognition intensity followed the order: HPV16 > HPV18 > HPV06 > HPV11.
- Achieved a limit of detection of 26 fM and a limit of quantification of 79.6 fM for the E6E7 microfluidic LOC-Genosensor.
Conclusions:
- The developed electrochemical genosensor offers a sensitive and specific platform for HPV detection.
- This technology can aid in the early diagnosis of HPV infections and the identification of oncogenic risk.
- The microfluidic LOC-Genosensor presents a valuable alternative for clinical HPV diagnosis.
