Protocol for hybrid structured-light three-dimensional scanning and modeling of human postmortem brains

Area of Science:

• Neuroscience
• Biomedical Engineering
• Medical Imaging

Background:

• Standardized protocols for sectioning postmortem human brain tissue are lacking.
• Current neuropathological evaluation methods lack systemic standards for tissue sectioning.

Purpose of the Study:

• To present a novel protocol for postmortem brain hybrid structured-light scanning.
• To facilitate the 3D printing of sectioning matrices for consistent tissue preparation.
• To streamline histopathology, volumetric analyses, and medical education applications.

Main Methods:

• Tissue preparation for 3D scanning.
• Hybrid structured-light scanning of postmortem brain tissue.
• 3D printing of custom sectioning matrices.
• Matrix-guided sectioning of brain tissue.

Main Results:

• A reproducible protocol for sectioning postmortem human brain tissue was developed.
• The protocol integrates 3D scanning and printing technologies.
• The method enables precise and consistent tissue sectioning.

Conclusions:

• The presented protocol offers a standardized approach to postmortem brain tissue sectioning.
• This method enhances the reliability of histopathology and volumetric analyses.
• The protocol has significant implications for medical education and research.