Silver-based bimetallic nanozyme fabrics with peroxidase-mimic activity for urinary glucose detection

Area of Science:

• Nanomaterials Science
• Catalysis
• Biomedical Engineering

Background:

• Bimetallic nanoparticles exhibit enhanced catalytic properties compared to monometallic counterparts.
• Peroxidase-mimicking nanozymes are crucial for biosensing applications.
• Developing stable and sensitive nanozyme-based sensors remains a challenge.

Purpose of the Study:

• To fabricate bimetallic Ag-M (M = Au, Pt, Pd) cotton fabrics with enhanced peroxidase-mimicking activity.
• To investigate the catalytic properties and radical generation of the fabricated nanozyme fabrics.
• To evaluate the Ag-Pt bimetallic nanozyme fabric as a sensor for direct urinary glucose detection.

Main Methods:

• Fabrication of bimetallic Ag-M cotton fabrics via electroless deposition and galvanic replacement.
• Assessment of peroxidase-mimicking catalytic activity and radical generation (hydroxyl and superoxide).
• Evaluation of the Ag-Pt nanozyme fabric sensor for direct urine glucose detection in the millimolar range.

Main Results:

• The Ag-Pt bimetallic nanozyme fabric demonstrated superior catalytic activity compared to Ag fabric.
• The Ag-Pt nanozyme fabric effectively generated both hydroxyl (•OH) and superoxide (O2•−) radicals.
• The sensor accurately detected high millimolar urinary glucose without sample predilution, matching clinical assay performance.
• The sensor showed robustness against interference from complex urinary matrices.

Conclusions:

• Bimetallic Ag-Pt nanozyme fabric exhibits significant peroxidase-mimicking activity and radical generation.
• The developed sensor enables direct, accurate, and interference-resistant detection of urinary glucose.
• This Ag-Pt nanozyme fabric sensor is a promising candidate for point-of-care urinary glucose monitoring.