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Updated: Jun 16, 2025

Visualizing Hyporheic Flow Through Bedforms Using Dye Experiments and Simulation
Published on: November 18, 2015
Relevant insights and concepts overlooked throughout the development of flow analysis. A tutorial
Valdemir A F Barros1, Elias A G Zagatto1
1Centre for Nuclear Energy in Agriculture, University of Sao Paulo, P.O. Box 96, Piracicaba, 13400-970, Brazil.
Abstract:
The conceptual expansion, fast development, and general acceptance of flow analysis are consequence of its adherence to the principles of green and white analytical chemistry, and chemical derivatization plays an essential role in this context. Through the flow analysis development, however, some of its potentialities and limitations have been overlooked. This is more evident when the involved modifications in flow rates, timing and/or manifold architecture deteriorate the analytical signals. These aspects have not always been systematically investigated, and are addressed here in relation to flow analyzers with UV-Vis spectrophotometric detection. Novel strategies for solution handling, guidance for dealing with the aforementioned analytical signal deterioration, and an alternative possibility for exploiting differential aspiration are presented. The concept of blank reagent carrier stream is proposed.
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