Nanoflower Microreactor Based Versatile Enhancer for Recognition Cofactor-Dependent Enzyme Biocatalysis toward
Liu-Na Wei1, Lin Luo1, Hong-Tao Lei1
1Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.
ACS Applied Materials & Interfaces
|August 21, 2024
Summary
This study developed a new alkaline phosphatase-antibody-CaHPO4 hybrid nanoflower (AAHNFs) microreactor for sensitive immunosensors. The AAHNFs enhance antibody stability and enable precise saxitoxin detection with a portable device.
Area of Science:
- Biomolecular Engineering
- Nanomaterials Science
- Analytical Chemistry
Background:
- Sensitive immunosensors are crucial for detecting analytes, but their performance is limited by biomolecule stability and signal amplification.
- Organic-inorganic hybrid nanoflowers offer potential for encapsulating biomolecules, yet their integration into functional immunosensors requires further investigation.
- Efficient utilization of organic carriers and bioactivity is key to developing advanced immunosensing platforms.
Purpose of the Study:
- To design and synthesize novel alkaline phosphatase-antibody-CaHPO4 hybrid nanoflowers (AAHNFs) for enhanced immunosensor applications.
- To investigate the factors influencing antibody recognition activity within the AAHNFs microreactor.
- To develop a sensitive and portable colorimetric immunosensor for the detection of saxitoxin (STX).
Main Methods:
- Fabrication of AAHNFs through a biomineralization process, integrating alkaline phosphatase (ALP) and antibodies.
- Investigation of AAHNFs' stability and antibody recognition capabilities under various conditions.
- Development of a colorimetric immunoprobe utilizing ALP activity for signal amplification and silver nanoplate localized surface plasmon resonance.
- Integration of the immunosensor with a 3D-printed portable device and smartphone sensing platform for data analysis.
Main Results:
- AAHNFs demonstrated enhanced protection of encapsulated antibodies against thermal and chemical degradation, preserving recognition ability.
- The developed colorimetric immunosensor achieved a sensitive detection range of 0.1-6.25 ng·mL⁻¹ for STX, with a low detection limit of 0.06 ng·mL⁻¹.
- Real sample analysis showed high average recoveries for STX, ranging from 85.9% to 105.9%, validating the sensor's practical applicability.
Conclusions:
- The AAHNFs microreactor effectively integrates enzymes and antibodies, offering improved stability and recognition for immunosensor development.
- The developed portable colorimetric immunosensor provides a sensitive and reliable method for saxitoxin detection.
- This study offers valuable insights into optimizing antibody performance in practical immunosensing applications.
