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High Performance Liquid chromatography - Fourier Transform Infrared Spectroscopy Coupling: A Comprehensive Review
Radoslav Halko1, Denis Pavelek1, Massoud Kaykhaii1,2
1Faculty of Natural Science, Department of Analytical Chemistry, Comenius University in Bratislava, Bratislava, Slovak Republic.
This review examines High-Performance Liquid Chromatography-Fourier Transform Infrared Spectrometry (HPLC-FTIR) interfaces since 2010. It highlights methods for analyzing complex mixtures with high sensitivity, addressing challenges like mobile phase interference.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Chromatography
Background:
- High-Performance Liquid Chromatography-Fourier Transform Infrared Spectrometry (HPLC-FTIR) coupling provides sensitive analysis of complex mixtures.
- Challenges include spectral distortion from mobile phase components.
Purpose of the Study:
- To critically review HPLC-FTIR interfaces developed since 2010.
- To identify key parameters for successful coupling and accurate analyte identification.
Main Methods:
- Review of literature on HPLC-FTIR interfaces and coupling strategies.
- Analysis of methods for solvent elimination and spectral data acquisition.
Main Results:
- Effective interfaces include flow cells and off-line methods (e.g., nebulizers).
- Key parameters influencing results are solvent elimination, FTIR mode, and mobile phase composition.
Conclusions:
- HPLC-FTIR is a powerful technique for qualitative and quantitative analysis.
- Optimizing interfaces and mobile phases is crucial for accurate identification of analytes in complex mixtures.
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