Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

2.3K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.3K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

2.7K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.7K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

1.9K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
1.9K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

2.5K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.5K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

2.6K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CD39 mRNA therapy attenuates localized acute inflammation: A novel anti-inflammatory strategy using cationic nanoliposomes.

Molecular therapy. Nucleic acids·2026
Same author

Platelet-Targeted Self-Amplifying mRNA for Safe, Long-Acting Thromboprophylaxis.

Circulation research·2026
Same author

Prothrombotic genetic variants and cancer-associated venous thromboembolism: defining thrombotic risk across tumor types.

Haematologica·2026
Same author

Harnessing Immune Pathways for Stroke Recovery: Overcoming Challenges to Clinical Translation.

Circulation research·2026
Same author

Innovative γ-Oryzanol and KC2 Based Lipid Nanoparticles: OryKL Platform Provides Safe and Efficient In Vivo mRNA Delivery.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Chondroitin Sulfate Enrichment in the Extracellular Matrix Inhibits NETosis in a 3D Calcific Aortic Valve Disease Model.

Advanced healthcare materials·2026

Related Experiment Video

Updated: Jun 15, 2025

Real-Time, High-Throughput Microscopic Quantification of Human Neutrophil Extracellular Trap Release and Assessing the Pharmacology of Antagonists
11:32

Real-Time, High-Throughput Microscopic Quantification of Human Neutrophil Extracellular Trap Release and Assessing the Pharmacology of Antagonists

Published on: October 18, 2024

568

Piezo1 expression in neutrophils regulates shear-induced NETosis.

Sara Baratchi1,2,3, Habiba Danish4, Chanly Chheang5

  • 1Baker Heart and Diabetes Institute, Melbourne, VIC, 3004, Australia. sara.baratchi@baker.edu.au.

Nature Communications
|August 22, 2024
PubMed
Summary

Shear stress activates the Piezo1 channel in neutrophils, triggering intracellular calcium increases and cytoskeleton changes that lead to neutrophil extracellular trap formation (NETosis), contributing to inflammation and thrombosis.

More Related Videos

High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro
11:03

High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro

Published on: June 18, 2016

13.1K
The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
09:20

The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress

Published on: October 31, 2016

8.1K

Related Experiment Videos

Last Updated: Jun 15, 2025

Real-Time, High-Throughput Microscopic Quantification of Human Neutrophil Extracellular Trap Release and Assessing the Pharmacology of Antagonists
11:32

Real-Time, High-Throughput Microscopic Quantification of Human Neutrophil Extracellular Trap Release and Assessing the Pharmacology of Antagonists

Published on: October 18, 2024

568
High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro
11:03

High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro

Published on: June 18, 2016

13.1K
The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
09:20

The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress

Published on: October 31, 2016

8.1K

Area of Science:

  • Immunology
  • Cell Biology
  • Biophysics

Background:

  • Neutrophil extracellular trap formation (NETosis) contributes to sterile inflammation and thrombosis.
  • Neutrophils experience hemodynamic forces like shear stress in circulation, influencing their functions.
  • Mechanisms of mechanotransduction in neutrophils remain incompletely understood.

Purpose of the Study:

  • To investigate the role of shear stress in neutrophil extracellular trap formation (NETosis).
  • To elucidate the molecular mechanisms underlying shear stress-induced NETosis in neutrophils.

Main Methods:

  • Exposure of neutrophils to varying levels of shear stress.
  • Measurement of intracellular calcium levels.
  • Assessment of Piezo1 channel and calpain activity.
  • Analysis of cytoskeleton remodeling and NETosis induction.

Main Results:

  • Shear stress induces NETosis in a level-dependent manner.
  • Shear stress enhances neutrophil sensitivity to NETosis-inducing agents.
  • Shear stress increases intracellular calcium via the mechanosensitive ion channel Piezo1.
  • Piezo1 activation mediates calpain activity and cytoskeleton remodeling, leading to NETosis.

Conclusions:

  • Shear stress is a direct inducer of NETosis in neutrophils.
  • The Piezo1-calpain-cytoskeleton axis is a key pathway for shear stress-mediated NETosis.
  • This pathway links mechanical forces to inflammatory and prothrombotic responses mediated by neutrophils.