A lumen-tunable triangular DNA nanopore for molecular sensing and cross-membrane transport

Area of Science:

• Biomolecular Engineering
• Nanotechnology
• Synthetic Biology

Background:

• DNA synthetic membrane nanopores show promise for molecular transport control.
• Achieving tunable lumen size in nanopores is a key challenge for biosensing and synthetic cells.

Purpose of the Study:

• To design and characterize a novel triangular DNA nanopore with a large, tunable lumen.
• To demonstrate controllable molecular transport through the DNA nanopore.

Main Methods:

• Design of a triangular DNA nanopore with specific DNA binding sites for mechanical control.
• Transmission electron microscopy (TEM) and molecular dynamics (MD) simulations for structural analysis.
• Single-channel current recordings and fluorescence influx studies for transport analysis.

Main Results:

• The triangular DNA nanopore exhibits stable architecture and high shape retention during lumen size transitions.
• In-situ transitions between expanded and contracted states were achieved via DNA binding stimuli.
• Low-noise, repeatable electrical readouts and controlled cross-membrane macromolecular transport were demonstrated.

Conclusions:

• The developed DNA nanopore offers a tunable lumen for precise control over molecular transport.
• This system provides a versatile platform for applications in molecular sensing, drug delivery, and synthetic cell development.