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Pathogenic Detection by Metagenomic Next-generation Sequencing in Skin and Soft Tissue Infection.
Ting-Kuang Yeh1,2, Yao-Ting Huang3, Po-Yu Liu1,2,4,5,6
1Division of Infectious Diseases, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan, R.O.C.
In Vivo (Athens, Greece)
|August 26, 2024
Summary
Metagenomic next-generation sequencing (mNGS) detects more pathogens in skin and soft tissue infections (SSTIs) than traditional cultures. This advanced method also identifies antimicrobial resistance genes, aiding treatment decisions.
Area of Science:
- Microbiology
- Infectious Diseases
- Genomics
Background:
- Skin and soft tissue infections (SSTIs) pose significant health risks.
- Conventional bacterial cultures for SSTIs have limitations in sensitivity and speed.
- Metagenomic next-generation sequencing (mNGS) offers a potential advancement in pathogen detection.
Purpose of the Study:
- To compare the efficacy of mNGS with conventional bacterial culture for pathogen detection in SSTIs.
- To evaluate the utility of mNGS in identifying diverse and rare pathogens in SSTIs.
- To assess the detection of antimicrobial resistance genes using mNGS in SSTI samples.
Main Methods:
- Prospective collection of 19 SSTI samples from 16 patients between January 2020 and October 2021.
- Simultaneous analysis of clinical samples using mNGS and conventional bacterial culture.
- Comparison of microbiological findings and analysis of clinical characteristics.
Main Results:
- mNGS identified a higher proportion of multiple pathogens compared to conventional culture.
- Commonly detected Gram-negative bacilli included Pseudomonas spp.
- Rare pathogens such as Granulicatella adiacens, Bacillus thuringiensis, and Bacteroides fragilis were identified by mNGS.
- Antimicrobial resistance genes were detected in 52.6% of samples, including those for ESBLs, CMY, and carbapenemases.
Conclusions:
- mNGS is a valuable tool for pathogen detection in SSTIs, offering broader identification than traditional methods.
- The detection of additional microbes and antimicrobial resistance genes by mNGS can inform clinical treatment decisions.
- Further research is needed to fully elucidate the comparative advantages of mNGS over conventional culture in SSTI management.
