Isothermal Technologies for HPV Detection: Current Trends and Future Perspectives.

Area of Science:

• Virology
• Molecular Diagnostics
• Public Health

Background:

• Human papillomavirus (HPV) comprises over 200 genotypes, with high-risk types linked to cancers like cervical cancer.
• Current HPV detection methods (cytology, hybrid capture, qPCR) face limitations in timely diagnosis.
• Cervical cancer remains a significant cause of mortality in women globally.

Purpose of the Study:

• To review isothermal nucleic acid amplification tests (INAATs) for HPV detection.
• To analyze INAAT characteristics: genotypes, targets, LOD, methods, and time.
• To discuss FDA-approved INAATs and challenges for widespread implementation.

Main Methods:

• Literature review of INAATs for HPV detection.
• Analysis of key performance metrics: HPV genotypes, gene targets, limit of detection (LOD).
• Evaluation of detection methodologies, time, and market-approved tests.

Main Results:

• INAATs provide advantages over qPCR, including reduced need for specialized labs and equipment.
• Specific INAATs show potential for rapid and sensitive HPV detection.
• A range of INAATs are being developed and evaluated for clinical use.

Conclusions:

• INAATs present a viable alternative for timely and accessible HPV detection.
• Addressing implementation challenges is crucial for leveraging INAATs in underserved areas.
• Further development and validation of INAATs can improve cervical cancer screening and prevention strategies.