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Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Related Experiment Video

Updated: Jun 12, 2026

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
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Maize mutant screens: from classical methods to new CRISPR-based approaches.

Christian Damian Lorenzo1,2, David Blasco-Escámez1,2, Arthur Beauchet1,2

  • 1Center for Plant Systems Biology, VIB, B-9052, Ghent, Belgium.

The New Phytologist
|August 30, 2024
PubMed
Summary

Mutations drive evolution and domestication in maize (Zea mays). Recent advances in genome editing (GE) using CRISPR-Cas technologies offer promising new avenues for maize trait engineering and future research.

Keywords:
CRISPRforward geneticsmaizemutantsreverse geneticsscreeningstransposons

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Area of Science:

  • Plant genetics
  • Genomics
  • Agricultural science

Background:

  • Maize (Zea mays) is a vital staple crop and a long-standing model organism for genetic research.
  • Mutations are fundamental to species evolution, domestication, and trait development.
  • The advent of site-directed mutagenesis and genome editing (GE) technologies has revitalized maize mutation research.

Purpose of the Study:

  • To review the progression and insights gained from maize screening studies.
  • To focus on recent genome editing (GE) approaches in maize.
  • To explore how different genetic strategies contribute to understanding maize trait variability.

Main Methods:

  • Analysis of forward genetics screens.
  • Examination of natural variation in maize populations.
  • Review of reverse genetics approaches.
  • Focus on recent genome editing (GE) techniques, particularly CRISPR-Cas.

Main Results:

  • Maize's genetic and physiological traits combined with CRISPR-Cas tools present significant opportunities for trait engineering.
  • Each genetic strategy (forward, natural variation, reverse) deepens the understanding of maize trait variability.
  • GE approaches are enhancing the ability to induce and analyze mutations.

Conclusions:

  • The integration of diverse genetic screening strategies and GE tools is crucial for advancing maize improvement.
  • Understanding induced and natural trait variability in maize is key to designing future mutational screenings.
  • CRISPR-based GE holds immense potential for accelerating the development of next-generation maize varieties.