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Published on: May 17, 2013
Preparing eugregarine parasites and their cricket host Acheta domesticus as a model for gregarine infection studies
Edouard Bessette1,2, Bryony A P Williams1, Nicolai V Meyling2
1Living Systems Institute, Department of Biosciences, Faculty of Health and Life Sciences, University of Exeter, Exeter, EX4 4QD, United Kingdom.
Abstract:
The increasing global demand for house crickets (Acheta domesticus) necessitates effective health measures. Despite their abundance, the effects of gregarines on these hosts remain underexplored. We present a method for controlled gregarine infection and maintenance of gregarine-free cricket populations. This system, adapted from prior work, is essential for experiments on gregarine infection dynamics, providing insights into parasite evolution and host-parasite interactions within the Apicomplexa group. This protocol includes rearing and maintenance of gregarine-free cricket populations for experimental purposes, gregarine production for oocyst solution and cricket infection, and gregarine infection assessment.

