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Related Experiment Videos

Lymphocyte labelling with indium: cytotoxicity studies.

N S Ghaleb, S Roath, P Wyeth

    European Journal of Nuclear Medicine
    |January 1, 1985
    PubMed
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    Indium In111 labelling of lymphocytes using oxine impairs cell viability and function. Alternative ligands like tropolone may reduce lymphocyte toxicity for reliable clinical assessment.

    Area of Science:

    • Nuclear medicine
    • Immunology
    • Cell biology

    Background:

    • Lymphocyte labelling with indium In111 is crucial for diagnostic imaging.
    • Assessing the impact of labelling on lymphocyte viability and function is essential for accurate clinical interpretation.

    Purpose of the Study:

    • To evaluate the cytotoxicity of indium In111 labelling on human lymphocytes.
    • To compare the toxicity of different ligands used in lymphocyte labelling.

    Main Methods:

    • Lymphocytes were labelled with indium In111 using oxine.
    • Cell viability was assessed using trypan-blue exclusion and rosetting assays.
    • Lymphocyte response to phytohaemagglutinin stimulation was measured by tritiated-thymidine uptake.

    Main Results:

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    • Indium In111 labelling with oxine significantly impaired lymphocyte viability and function.
    • Impairment was observed even at labelling levels suitable for imaging.
    • Tropolone as a ligand showed potential for reduced cellular toxicity compared to oxine.

    Conclusions:

    • Indium In111 labelling, particularly with oxine, induces cytotoxicity in human lymphocytes.
    • The prolonged lifespan of lymphocytes makes them susceptible to labelling-induced damage, potentially compromising long-term clinical studies.
    • Exploring alternative ligands like tropolone is recommended to improve the reliability of lymphocyte-based diagnostic assessments.