Related Experiment Video
Updated: Jul 18, 2026
09:42
Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining
Published on: December 23, 2014
21.2K
Periodic Acid Schiff Staining to Detect Mott Cells, Aberrant Plasma Cells Containing Immunoglobulin Inclusions Called
Pedram Mahmoudi Aliabadi1, Khlowd Al-Qaisi1, Vishnu Reddy2
1Deutsches Rheuma-Forschungszentrum Berlin, Berlin, Germany.
Current Protocols
|September 4, 2024
Summary
This study presents a protocol using periodic acid Schiff (PAS) staining to detect Mott cells, which are plasma cells with immunoglobulin inclusions. This method is effective across various sample types for histopathological analysis.
Area of Science:
- Histopathology
- Immunology
- Cell Biology
Background:
- Hematoxylin and eosin (H&E) staining is standard for routine histopathology.
- Periodic acid Schiff (PAS) staining detects polysaccharides and is crucial in immunology for evaluating kidney lesions.
- PAS staining does not stain erythrocytes, aiding immune cell identification in the spleen.
Purpose of the Study:
- To describe a protocol for detecting Mott cells, characterized by immunoglobulin inclusion bodies (Russell bodies).
- To provide a reliable method for identifying these specific plasma cell alterations.
Main Methods:
- Utilizes periodic acid Schiff (PAS) staining.
- Applicable to formalin-fixed, paraffin-embedded tissue sections.
- Also effective for frozen tissue sections, touch preparations, blood films, and cytocentrifuged cell smears.
Main Results:
- Successful detection of Mott cells using the described PAS staining protocol.
- Demonstrates the versatility of the protocol across different sample preparations.
Conclusions:
- The PAS staining protocol provides an effective means for identifying Mott cells.
- This method is adaptable for various histopathological and cytological sample types.
Related Concept Videos
01:26Differential Staining Technique
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
01:13Special Staining Techniques
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...