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Published on: May 2, 2018
Sampling the Maize (Zea mays) Leaf Microbiome
Jason G Wallace1,2,3
1Department of Crop and Soil Science, University of Georgia, Athens, Georgia 30602, USA jason.wallace@uga.edu.
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The microbiota of maize leaves can be beneficial or detrimental to the host. Foliar diseases are the most obvious detrimental impact of the leaf microbiome, though more subtle effects of the normal (nondisease) community are an active area of research. This protocol describes two specific methodologies to sample the maize leaf microbiome: one sampling the surface (epiphyte) microbiome and one sampling the interior (endophyte) microbiome. Each method begins with collected leaf tissue and finishes with a cell suspension suitable for either isolating live microbes or extracting DNA for sequencing.

