Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Seed Structure and Early Development of the Sporophyte

Seed Structure and Early Development of the Sporophyte

Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Sampling the Maize (<i>Zea mays</i>) Leaf Microbiome.

Cold Spring Harbor protocols·2024

Same author

Preparation of Illumina 16s Amplicon Sequencing Libraries with Peptide Nucleic Acids (PNAs) for the Analysis of Maize-Associated Microbiomes.

Cold Spring Harbor protocols·2024

Same author

Sampling Root-Associated Microbiome Communities of Maize (<i>Zea mays</i>).

Cold Spring Harbor protocols·2024

Same author

Sampling and Analysis of the Maize Microbiome.

Cold Spring Harbor protocols·2024

Same author

Manipulating the Maize (<i>Zea mays</i>) Microbiome.

Cold Spring Harbor protocols·2024

Same author

Corrigendum: Mining the <i>Utricularia gibba</i> genome for insulator-like elements for genetic engineering.

Frontiers in plant science·2024

Same journal

High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

Cold Spring Harbor protocols·2025

Same journal

Grain Quality in Maize.

Cold Spring Harbor protocols·2025

Same journal

High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

Cold Spring Harbor protocols·2025

Same journal

Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

Cold Spring Harbor protocols·2025

Same journal

The UniformMu National Public Resource: Transposon<i>-</i>Induced Mutant Seeds for Functional Genomics Studies in Maize.

Cold Spring Harbor protocols·2025

Same journal

Insights from the Study of B<i>-</i>Cell Epitopes of a Microbial Pathogen by Phage Display.

Cold Spring Harbor protocols·2025

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 14, 2025

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins

Published on: February 8, 2019

Sampling Maize (Zea mays) Seed Endophytes.

Jason G Wallace^1,2,3, Daniel Laspisa^2,3

¹Department of Crop and Soil Science, University of Georgia, Athens, Georgia 30602, USA jason.wallace@uga.edu.

Cold Spring Harbor Protocols

|September 5, 2024

Summary

This summary is machine-generated.

This study details a method for isolating maize seed endophytes, focusing on surface sterilization and grinding. Understanding these microbes is crucial for seed health and future plant development.

More Related Videos

Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control

Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control

Published on: April 11, 2013

Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize

Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize

Published on: September 15, 2023

Related Experiment Videos

Last Updated: Jun 14, 2025

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins

Published on: February 8, 2019

Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control

Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control

Published on: April 11, 2013

Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize

Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize

Published on: September 15, 2023

Area of Science:

Plant Science
Microbiology
Agricultural Science

Background:

Maize grain production is a primary agricultural goal.
Plant seeds, especially maize, are key for vertical microbial transmission.
The role of seed endophytes in plant health is largely unknown.

Purpose of the Study:

To describe a protocol for isolating seed endophytes from maize.
To enable further research into the function of these endophytes.

Main Methods:

The protocol emphasizes thorough seed surface sterilization.
Seed grinding is used to release internal endophytes.
The resulting cell suspension is prepared for culturing or DNA analysis.

Main Results:

A reliable method for isolating maize seed endophytes was established.
The protocol yields a cell suspension suitable for downstream applications.

Conclusions:

This protocol facilitates the study of maize seed endophytes.
Further research can now investigate the impact of endophytes on seed health and plant generation.