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Updated: Jun 14, 2025

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
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Improved Yield of SPRI Beads-Based Size Selection in the Very High Molecular Weight Range

Alexei Stortchevoi1, Stuart S Levine1

  • 1BioMicro Center Department of Biology, Massachusetts Institute of Technology CambridgeMA.

Journal of Biomolecular Techniques : JBT
|September 6, 2024
PubMed
Abstract

No abstract available in PubMed .

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Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
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