Glucose Oxidase-Based Glucose Biosensing with a Simple Dual Ag/AgCl Probe Conductivity Readout
Kawin Loha1, H Sasimali M Soysa1,2, Thitikorn Boonkoom3
1School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wang Chan Valley, Rayong 21210, Thailand.
Analytical Chemistry
|September 12, 2024
Summary
This study presents a simple conductometric assay for glucose detection using glucose oxidase (GOx). The method avoids complex designs, offering a scalable and affordable approach for glucose analysis in various samples.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Biosensing Technology
Background:
- Electrochemical glucose biosensors often rely on complex designs, enzyme immobilization, and nanomaterials.
- Existing methods can be costly and intricate, limiting widespread application.
- A need exists for simpler, more accessible glucose detection strategies.
Purpose of the Study:
- To develop a simplified conductometric assay for glucose detection.
- To utilize basic ionic conductivity principles for glucose measurement.
- To avoid complex sensor designs and enzyme immobilization techniques.
Main Methods:
- A conductometric assay employing dissolved glucose oxidase (GOx) was developed.
- The assay measures ionic conductivity changes resulting from glucose conversion to gluconic acid.
- A silver/silver chloride wire pair and a small applied potential were used to generate a measurable current.
Main Results:
- The assay demonstrated linearity up to 500 μM glucose.
- A sensitivity of approximately 1.3 nA/μM was achieved.
- High selectivity for glucose over other sugars and accurate quantification in a model sample and beverage were observed.
Conclusions:
- The proposed non-amperometric glucose biosensing strategy is simple and effective.
- The assay avoids complex sensor fabrication, offering a potentially affordable solution.
- Feasible integration into planar electrode platforms suggests broad applicability for glucose analysis.
