Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Downstream Processing01:29

Downstream Processing

Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bifidobacterial genes upregulated by resistant starch investigated using multi-omics have orthologs in infant gut isolates.

ISME communications·2026
Same author

Unveiling Solvent-Mediated Mechanochemical Cocrystallization Pathways by <i>In Situ</i> CLASSIC NMR Spectroscopy.

Molecular pharmaceutics·2026
Same author

A 5-Br-1-Propylisatin Derivative as a Promising BRD9 Ligand: Insights from Computational and STD NMR Investigation.

Molecules (Basel, Switzerland)·2026
Same author

The impact of freezing temperatures on soft tissue and microbial decomposition using human and porcine remains: a pilot study.

Forensic science, medicine, and pathology·2025
Same author

Fast and Reliable NMR-Based Fragment Scoring for Drug Discovery.

Journal of the American Chemical Society·2025
Same author

Mineral Speciation for CO<sub>2</sub> Captured by Potassium Hydroxide.

ChemistryOpen·2025

Related Experiment Video

Updated: Jun 13, 2026

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications
08:50

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

Published on: August 4, 2017

6.7K

Probing assembly/disassembly of ordered molecular hydrogels.

Susana M Ramalhete1, Karol P Nartowski1,2, Hayley Green3

  • 1School of Pharmacy, University of East Anglia, Norwich Research Park, Norwich, NR2 1TS, UK. Y.Khimyak@uea.ac.uk.

Faraday Discussions
|September 18, 2024
PubMed
Summary

This study explores how modifying amino acids like L-Phenylalanine (Phe) affects hydrogel properties. Researchers used NMR crystallography to understand interactions in multi-component gels, revealing insights into self-assembly and material functionality.

More Related Videos

An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components
08:17

An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components

Published on: July 18, 2018

7.1K
Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility
05:24

Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility

Published on: September 6, 2024

1.1K

Related Experiment Videos

Last Updated: Jun 13, 2026

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications
08:50

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

Published on: August 4, 2017

6.7K
An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components
08:17

An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components

Published on: July 18, 2018

7.1K
Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility
05:24

Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility

Published on: September 6, 2024

1.1K

Area of Science:

  • Supramolecular chemistry
  • Materials science
  • Biomedical engineering

Background:

  • Supramolecular hydrogels are vital in biomedical applications, including tissue engineering and drug delivery.
  • L-Phenylalanine (Phe) self-assembles into fibrous networks, with modifications influencing gelation.
  • Controlling multi-component gel systems offers advantages for tuning material properties.

Purpose of the Study:

  • To investigate the structural and dynamic properties of multi-component hydrogels formed by Phe and amino-L-phenylalanine (NH2-Phe).
  • To explore the patterns of ordered/disordered domains within gel fibers and their interfaces.
  • To elucidate the general trends of intermolecular interactions in these complex hydrogel systems.

Main Methods:

  • Utilized multicomponent gels based on Phe and NH2-Phe.
  • Employed NMR crystallography (combining solid- and solution-state NMR) for structural and dynamic insights.
  • Supported findings with experimental (diffraction, rheology, microscopy, thermal analysis) and computational methods.

Main Results:

  • Phe and NH2-Phe self-assemble into crystalline hydrogels in water.
  • NH2-Phe exhibited faster exchange dynamics than Phe, correlating with weaker intermolecular interactions.
  • Low NH2-Phe concentrations disrupted the network by interfering with Phe's electrostatic interactions; high concentrations formed novel multi-gelator hydrogels.

Conclusions:

  • The head groups of amino acids play a crucial role in dictating the strength of intermolecular interactions in hydrogels.
  • Structural and dynamic heterogeneities in hydrogels can be effectively probed using advanced NMR techniques.
  • Multi-component hydrogel systems provide a versatile platform for tailoring material properties for specific applications.