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Bidirectional in-silico clearing approach for deep refractive-index tomography using a sparsely sampled transmission
Osamu Yasuhiko1, Kozo Takeuchi1
1Central Research Laboratory, Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamana-ku, Hamamatsu, Shizuoka 434-8601, Japan.
Biomedical Optics Express
|September 19, 2024
Summary
This study introduces bidirectional in-silico clearing refractive index tomography (ODT) to overcome scattering limitations in biological imaging. The novel technique enhances imaging depth for label-free volumetric analysis of complex biological samples.
Area of Science:
- Biophotonics
- Optical imaging
- Tomography
Background:
- Optical diffraction tomography (ODT) provides label-free volumetric imaging by mapping refractive index (RI) distributions.
- Imaging depth in ODT is limited by multiple scattering from inhomogeneous RI in biological specimens.
Purpose of the Study:
- To develop a novel ODT technique for enhanced imaging depth in biological samples.
- To overcome the limitations imposed by multiple scattering in thick specimens.
Main Methods:
- Introduced bidirectional in-silico clearing RI tomography, incorporating forward and reversed in-silico clearing.
- Integrated ODT reconstruction with a transmission matrix approach for RI reconstruction and wave backpropagation.
- Utilized a sparsely sampled transmission matrix to reduce measurement and computational requirements.
Main Results:
- Successfully imaged a spheroid with a thickness of 263 µm (11.4 scattering mean free paths).
- Demonstrated consistent imaging performance across various biological specimens, including liver and colon spheroids.
- Achieved enhanced imaging depth without modifying conventional ODT setups.
Conclusions:
- Bidirectional in-silico clearing RI tomography significantly extends imaging depth in ODT.
- The method offers a versatile and computationally efficient solution for label-free volumetric imaging of thick biological samples.
- This technique holds promise for advanced studies of complex biological structures.
