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Related Experiment Video

Updated: Jun 12, 2025

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model
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Tissue-Engineered Oral Epithelium for Dental Material Testing: Toward In Vitro Biomimetic Models.

Foteini Machla1, Paraskevi Kyriaki Monou2,3, Chrysanthi Bekiari4

  • 1Department of Prosthodontics, Tissue Engineering Core Unit, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.

Tissue Engineering. Part C, Methods
|September 20, 2024
PubMed
Summary

Tissue-engineered oral epithelium (TEOE) was developed using air-liquid interface culture. This model effectively mimics oral tissue and serves as a valuable tool for assessing dental material biocompatibility.

Keywords:
biocompatibility assessment toolepithelial barrierin vitro–in vivo extrapolationoral mucosapermeabilitytissue engineering

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Oral Biology

Background:

  • Developing functional tissue-engineered oral epithelium (TEOE) is crucial for biocompatibility testing of dental materials.
  • Optimizing culture conditions is essential for creating TEOE that mimics native oral mucosa.

Purpose of the Study:

  • To develop and characterize TEOE using different culture conditions.
  • To evaluate the biocompatibility of dental resinous monomers using the developed TEOE model.

Main Methods:

  • Comparison of submerged (SUB) and air-liquid interface (ALI) culture methods.
  • Assessment of barrier function using transepithelial electrical resistance (TEER) and calcein permeation.
  • Evaluation of cell viability, tissue architecture, intercellular connections, and pancytokeratin expression.
  • Exposure of TEOE to dental resinous monomers to assess effects on viability and permeability.

Main Results:

  • Air-liquid interface/keratinocyte growth factor-supplemented (ALI-KGS) culture produced an 8-20 layer thick epithelial barrier.
  • ALI-KGS TEOE showed decreased TEER compared to other conditions but intensive pancytokeratin expression.
  • Dental monomers altered TEOE integrity and architecture, causing hydropic degeneration, but did not significantly affect permeability.

Conclusions:

  • ALI-KGS culture conditions effectively generate TEOE with physiological characteristics.
  • TEOE serves as a valuable in vitro model for assessing the biocompatibility of dental materials.
  • The TEOE model demonstrates resilience in barrier function despite monomer-induced structural changes.