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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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Related Experiment Video

Updated: Jun 12, 2025

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

Genetically Expressed RNA Strand Displacement for Cellular Manipulation.

Chao-Qun Wu¹, Hui-Ye Feng¹, Yan Liu²

¹MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China.

Chembiochem : a European Journal of Chemical Biology

|September 21, 2024

Summary

Genetically expressed RNA strand displacement enables sophisticated synthetic gene circuits in living cells. This technology offers stable, long-term regulation of biological functions by integrating with the genome.

Keywords:

Cellular Manipulation RNA Circuit RNA Strand Displacement Synthetic Biology

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Published on: May 29, 2020

Area of Science:

Molecular Biology
Synthetic Biology
Biotechnology

Background:

Nucleic acid strand displacement is fundamental to dynamic nanotechnologies.
RNA strand displacement offers advantages over DNA for in-cell applications.
Genetic expression allows seamless integration into cellular networks.

Purpose of the Study:

To review developments in synthetic gene circuits using RNA strand displacement.
To highlight the advantages of genetically expressed RNA systems.
To discuss future prospects and challenges in this field.

Main Methods:

Overview of previous research on RNA strand displacement in gene circuits.
Discussion of synthetic gene circuit designs.
Analysis of genetically expressed RNA systems.

Main Results:

Genetically expressed RNA strand displacement facilitates stable, long-term regulation within cellular networks.
This technology enables seamless integration into genomic networks.
Diverse biological functionalities can be regulated.

Conclusions:

Genetically expressed RNA strand displacement is a powerful tool for in vivo synthetic biology.
It offers unique advantages for stable and integrated gene circuit applications.
Further research is needed to address challenges and explore potential applications.