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Related Experiment Videos

Bacteria beneath composite restorations--a culturing and histobacteriological study.

B Mejàre, I Mejàre, S Edwardsson

    Acta Odontologica Scandinavica
    |January 1, 1979
    PubMed
    Summary

    Microbial flora under composite fillings was studied using anaerobic techniques. Cavity liners significantly reduced bacterial growth, suggesting improved oral health outcomes.

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    Unrestored dentin caries and deep dentin restorations in Swedish adolescents.

    Caries research·2008

    Area of Science:

    • Microbiology
    • Restorative Dentistry
    • Oral Health

    Background:

    • Understanding the microbial environment beneath dental restorations is crucial for assessing their long-term success.
    • Previous studies have investigated microbial flora in various oral conditions, but specific data on composite fillings with and without liners is limited.

    Purpose of the Study:

    • To investigate the occurrence, viability, and identification of microbial flora under composite fillings.
    • To compare the microbial flora in cavities treated with a cavity liner versus those without.

    Main Methods:

    • Class V cavities were prepared in premolars from children aged 11-15.
    • Teeth were randomly assigned to receive either a composite filling alone or a cavity liner followed by a composite filling.
    • Samples from the pulpal wall under fillings were analyzed using anaerobic culturing and histobacteriological techniques after 4-6 weeks.

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    Main Results:

    • No microbial growth was observed in cultures from lined cavities.
    • Significant microbial growth occurred in 6 out of 7 unlined cavities.
    • Histobacteriological examinations confirmed the presence or absence of microorganisms, showing full agreement with culturing results.

    Conclusions:

    • Cavity liners effectively inhibit the occurrence and viability of microbial flora under composite fillings.
    • The dominant microbial flora under unlined fillings consisted of Gram-positive bacteria (Streptococcus, Actinomyces) and Gram-negative bacteria (Veillonella, Fusobacterium, Campylobacter, Selenomonas).
    • The identified microbial flora composition resembles that of dental plaque more than carious dentin or saliva.