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[Efficient synthesis of salidroside from tyrosol based on UDPG recycling system]
Chenyu Wei1, Zhuying Huang1, Zhixing Shen1
1Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|September 25, 2024
Summary
This study developed a biosynthetic method for producing salidroside using engineered *Escherichia coli*. The engineered microbes achieved an 8.17 g/L yield, offering an efficient alternative to plant extraction for this valuable compound.
Area of Science:
- Biotechnology
- Synthetic Biology
- Metabolic Engineering
Background:
- Salidroside is a valuable compound with applications in food and pharmaceuticals.
- Traditional plant extraction methods for salidroside are limited by low yields and complex processes.
Purpose of the Study:
- To develop an efficient biosynthetic pathway for salidroside production in microorganisms.
- To optimize the microbial strain and fermentation conditions for high-yield salidroside synthesis.
Main Methods:
- Engineered *Escherichia coli* BL21/pETDuet-AtSUS-UGT33 strain construction using glycosyltransferase (UGT33) and sucrose synthase (AtSUS).
- Optimization of gene copy number ratio, whole-cell transformation conditions (temperature, pH, inoculum, substrate, metal ions), and fermentation parameters.
- Biosynthesis of salidroside from tyrosol using a uridine diphosphate glucose (UDPG) recycling system.
Main Results:
- Screening and selection of key enzymes AtSUS and UGT33 for efficient tyrosol glycosylation.
- Optimization of gene copy number ratio to 3:1 (UGT33:AtSUS) for maximal salidroside production.
- Achieved a highest salidroside yield of 8.17 g/L in a 5 L fermenter after 24 hours of fermentation under optimized conditions.
Conclusions:
- The developed microbial biosynthesis platform offers an efficient and scalable method for salidroside production.
- This study provides a valuable reference for the microbial production of salidroside and other glycosylated natural products.
- Biosynthesis presents a sustainable alternative to conventional plant extraction for obtaining functional ingredients like salidroside.
