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Updated: Jun 12, 2025
Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of GoldIII
Published on: August 31, 2018
Albumin host for supramolecular fluorescence recognition
Immanuel David Charles1, Lei Wang1, Yu Chen2
1Guangdong Provincial Key Laboratory of New Energy Materials Service Safety, College of Material Science and Engineering, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, P. R. China. bliu@szu.edu.cn.
Albumin-based supramolecular fluorescent indicator displacement assays (AS-FIDAs) offer sensitive detection of diverse analytes. These albumin (ALB)-based sensors are versatile tools for real-time monitoring in biological and environmental settings.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Environmental Science
Background:
- Synthetic molecular sensors are vital for real-time monitoring in complex biological and environmental systems.
- Detecting specific targets amidst interferents is challenging, especially in environments lacking reactive functional groups.
- Fluorescent sensors, particularly albumin-based supramolecular fluorescent indicator displacement assays (AS-FIDAs), have emerged as sensitive and rapid analytical tools.
Purpose of the Study:
- To review recent advancements in albumin (ALB)-based supramolecular sensors and sensor arrays.
- To highlight the application of AS-FIDAs in detecting a wide range of biologically and environmentally significant molecules.
- To showcase the versatility of AS-FIDAs in various analytical and diagnostic applications.
Main Methods:
- Utilizing albumin (ALB) as a versatile host molecule with multiple binding pockets for guest dye complexation.
- Developing ALB@dye ensembles for tailored analyte recognition through fluorescent indicator displacement.
- Exploring sensor arrays for simultaneous detection of multiple analytes.
Main Results:
- Demonstrated the practical utility and broad applicability of various AS-FIDAs.
- Pioneered the use of albumin as a host molecule in synthetic chemical sensors.
- Expanded AS-FIDA applications to include pesticides, hormones, biomarkers, drugs, and carcinogens.
Conclusions:
- AS-FIDAs represent a significant advancement in synthetic molecular sensing technology.
- The versatility of ALB-based supramolecular sensors makes them valuable for diverse settings, from lab research to portable devices.
- AS-FIDAs enable on-site monitoring, cell imaging, and real sample analysis, offering practical solutions for environmental and biological detection.
