Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

88
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
88
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

77
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
77

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A portable Cas6f-based system for multiplex translational repression in bacteria.

Nature communications·2026
Same author

Mind the Porins: Differential Effects of Porin Knockouts and Overexpression on Glucose and Xylose Uptake and Utilization in Pseudomonas putida.

Microbial biotechnology·2026
Same author

Amidase-Catalyzed Desorption of CO<sub>2</sub> Captured in Aqueous Monoethanolamine (MEA) Solutions.

Angewandte Chemie (International ed. in English)·2026
Same author

Adaptive Laboratory Evolution Unlocks Membrane Permeability as a Key Limitation in Long-Chain Alcohol Metabolism by Pseudomonas putida KT2440.

Microbial biotechnology·2026
Same author

Integrated control of redox and energy metabolism by the membrane-bound and soluble transhydrogenases of Pseudomonas putida across metabolic regimes.

Cell systems·2026
Same author

Cascading recombinase memory switch for programmable and stable gene expression in Pseudomonas putida.

Nucleic acids research·2026

Related Experiment Video

Updated: May 3, 2026

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

10.5K

A versatile microbial platform as a tunable whole-cell chemical sensor.

Javier M Hernández-Sancho1, Arnaud Boudigou1, Maria V G Alván-Vargas1

  • 1The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark.

Nature Communications
|September 27, 2024
PubMed
Summary

Researchers engineered a new whole-cell biosensor using Pseudomonas putida for detecting diverse chemicals. This adaptable microbial system offers sensitive, rapid monitoring for industrial microbiology and environmental analysis.

More Related Videos

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.9K
In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays
10:05

In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays

Published on: September 20, 2021

2.3K

Related Experiment Videos

Last Updated: May 3, 2026

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

10.5K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.9K
In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays
10:05

In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays

Published on: September 20, 2021

2.3K

Area of Science:

  • Synthetic Biology
  • Microbial Engineering
  • Chemical Sensing

Background:

  • Traditional biosensors often require engineered transcription factors, limiting their scope and applicability.
  • Industrial microbiology demands sensitive, specific, and portable tools for chemical detection and quantification.

Purpose of the Study:

  • To develop a versatile whole-cell biosensor platform for detecting a wide range of chemicals.
  • To overcome limitations of traditional biosensors by engineering Pseudomonas putida.
  • To enable real-time monitoring of industrial processes and environmental samples.

Main Methods:

  • Engineered Pseudomonas putida with integrated fluorescent reporters and synthetic auxotrophies.
  • Utilized growth-coupled setups where target analytes complement metabolic auxotrophies.
  • Demonstrated sensing in co-cultures and for complex biochemical processes like plastic degradation.

Main Results:

  • Successfully engineered a platform for whole-cell sensing of structurally diverse chemicals.
  • The biosensor demonstrated high sensitivity and adaptability across various conditions.
  • Applicable for monitoring plastic degradation by enzymes and engineered bacteria.

Conclusions:

  • This engineered microbial system provides a rapid, sensitive, and adaptable tool for cell factory monitoring.
  • The versatile biosensor platform is suitable for environmental analyses and industrial microbiology applications.
  • Offers a novel approach to overcome traditional biosensor limitations.