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Lactobacilli and Bifidobacteria: A Parapostbiotic Approach to Study and Explain Their Mutual Bioactive Influence
Clelia Altieri1, Alfonso Filippone1,2, Antonio Bevilacqua1
1Department of Agriculture, Food, Natural Resources and Engineering, University of Foggia, 71122 Foggia, Italy.
Foods (Basel, Switzerland)
|September 28, 2024
Summary
This study reveals that beneficial bacteria, Lactiplantibacillus plantarum and Bifidobacterium species, produce factors that influence each other's growth and survival. These findings highlight cross-kingdom interactions in microbial communities.
Area of Science:
- Microbiology
- Probiotics Research
- Bacterial Interactions
Background:
- Lactiplantibacillus plantarum and Bifidobacterium species are key probiotics.
- Understanding inter-species communication is crucial for probiotic efficacy.
- Cell-free supernatants (CFS) contain bioactive molecules influencing bacterial behavior.
Purpose of the Study:
- To investigate the influence of CFS from L. plantarum on Bifidobacterium growth and vice versa.
- To quantify the impact of these interactions on bacterial viability and stability.
- To identify potential bifidogenic and bioactive factors exchanged between these genera.
Main Methods:
- Cultivation of L. plantarum and Bifidobacterium strains.
- Preparation and application of cell-free supernatants (CFS) in growth media.
- Viable count assessment and modeling using a reparametrized Gompertz equation.
- Statistical analysis using multiparametric ANOVA.
Main Results:
- Evidence of bifidogenic/bioactive factors produced by Bifidobacterium influencing L. plantarum, and vice versa.
- Interactions affect bacterial growth, senescence, and death phases (stimulation and protection).
- Effects demonstrate strong strain and species-specific dependence.
Conclusions:
- Bifidobacteria and L. plantarum exhibit mutualistic interactions mediated by secreted factors.
- These interactions can enhance probiotic performance through growth stimulation and survival protection.
- Further research is needed to elucidate the specific mechanisms of these bioactive factors.
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