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Updated: May 3, 2026
11:07
Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging
Published on: November 24, 2021
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Characterisation of skin penetration pathways using stimulated Raman scattering microscopy
Anukrati Goel1, Ruth Pendlington2, Stephen Glavin2
1School of Chemistry and Chemical Engineering, University of Surrey, Guildford GU2 7XH, UK.
Summary
This study reveals how molecule lipophilicity dictates topical penetration into skin layers. Hydrophilic substances favor corneocyte cells, while lipophilic ones prefer the intercellular lipid matrix for effective topical delivery.
Area of Science:
- Dermatology
- Materials Science
- Biophysics
Background:
- Understanding skin penetration is vital for topical drug delivery and skincare.
- The skin's complex structure presents barriers to effective topical product formulation.
Purpose of the Study:
- To investigate the partitioning behavior of model permeants within human skin.
- To elucidate the relationship between molecular properties and skin penetration pathways.
Main Methods:
- Utilized stimulated Raman scattering (SRS) microscopy for label-free, chemical imaging.
- Assessed partitioning of six model permeants with varying lipophilicity (logP values).
- Analyzed penetration through intercellular lipid matrix and intracellular corneocyte routes.
Main Results:
- Molecular lipophilicity strongly influences partitioning into skin.
- Lipophilic compounds preferentially partitioned into the intercellular lipid matrix.
- Hydrophilic molecules showed increased affinity for corneocyte cells.
Conclusions:
- Findings clarify mechanisms of topical substance penetration based on lipophilicity.
- Provides insights for developing safer and more effective topical drugs and skincare.
- Offers advanced imaging methods for assessing drug delivery in healthy and diseased skin.
Keywords:
Cutaneous absorptionRaman spectroscopyStimulated Raman scattering microscopyTopical drug deliveryMore Related Videos
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