Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

5.7K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
5.7K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.1K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanical Stiffening Promotes Growth, Invasion-Associated Phenotypes, and Reduced Selumetinib Sensitivity in 3D Plexiform Neurofibroma Cultures.

Cells·2026
Same author

A positive feedback loop between BACH1 and IL-1β promotes the progression of HPV-negative head and neck squamous cell carcinoma.

Cell communication and signaling : CCS·2026
Same author

Exploring AI-assisted cameras to assess use of contact precautions.

Infection control and hospital epidemiology·2026
Same author

Mechanical Stiffening Promotes Growth, Invasion, and Mitogen-Activated Protein Kinase Kinase (MEK) Inhibitor Resistance in 3D Plexiform Neurofibroma Cultures.

bioRxiv : the preprint server for biology·2026
Same author

Cell viscosity influences haematogenous dissemination and metastatic extravasation of tumour cells.

Nature materials·2026
Same author

Brillouin Microscopy of Breast tumor Spheroids On-a-Chip: Mechanical and Transcriptional Responses to Microfluidic-Induced Rapid Deformations.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same journal

Ptychography at all wavelengths.

Nature reviews. Methods primers·2026
Same journal

Droplet-based bioprinting.

Nature reviews. Methods primers·2026
Same journal

Laser capture microdissection.

Nature reviews. Methods primers·2026
Same journal

Extracellular vesicle analysis.

Nature reviews. Methods primers·2026
Same journal

In vivo microelectrode arrays for neuroscience.

Nature reviews. Methods primers·2026
Same journal

Light-based vat-polymerization bioprinting.

Nature reviews. Methods primers·2025
See all related articles

Related Experiment Video

Updated: Jun 10, 2025

Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy
07:19

Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy

Published on: September 15, 2016

10.4K

Brillouin microscopy.

Irina Kabakova1, Jitao Zhang2, Yuchen Xiang3

  • 1School of Mathematical and Physical Sciences, University of Technology Sydney, Sydney, New South Wales, Australia.

Nature Reviews. Methods Primers
|October 11, 2024
PubMed
Summary
This summary is machine-generated.

Brillouin microscopy, a label-free imaging technique, has rapidly advanced over 20 years. It now offers high-resolution characterization of cell and tissue biomechanics, crucial for understanding disease.

More Related Videos

High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis
13:31

High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

Published on: December 22, 2015

15.0K
High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
07:55

High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis

Published on: September 22, 2017

10.1K

Related Experiment Videos

Last Updated: Jun 10, 2025

Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy
07:19

Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy

Published on: September 15, 2016

10.4K
High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis
13:31

High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

Published on: December 22, 2015

15.0K
High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
07:55

High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis

Published on: September 22, 2017

10.1K

Area of Science:

  • Optics and Photonics
  • Biophysics
  • Biomedical Imaging

Background:

  • Brillouin microscopy emerged ~20 years ago with high-resolution spectrometers.
  • The field has rapidly expanded, integrating diverse technologies like machine learning and quantum optics.
  • Advancements have significantly improved imaging speed, spectral resolution, and sensitivity.

Purpose of the Study:

  • To provide a comprehensive overview of Brillouin microscopy methods and applications.
  • To serve as a reference for researchers in the field.
  • To highlight the transformative potential of Brillouin microscopy in biology and biomedicine.

Main Methods:

  • Development of non-scanning high-resolution optical spectrometers.
  • Integration of technologies from telecommunications, astrophotonics, multiplexed microscopy, quantum optics, and machine learning.
  • Label-free and contact-free optical techniques for mechanical property characterization.

Main Results:

  • Significant improvements in imaging speed, spectral resolution, and sensitivity.
  • Enabling characterization of mechanical properties at cellular and subcellular scales.
  • Demonstrating the critical role of local biomechanics in predicting cellular fate and tissue pathogenesis.

Conclusions:

  • Brillouin microscopy is a rapidly evolving field with broad applications in biology and medicine.
  • Understanding cellular biomechanics is vital for disease research.
  • Future developments promise further transformative impacts in life sciences.