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Updated: Jun 12, 2025
Electromagnetic Controlled Closed-Head Model of Mild Traumatic Brain Injury in Mice
Published on: September 28, 2022
Protocol for inducing varying TBI severity in a mouse model using a closed-head, weight-drop, impact-induced
Javier Allende Labastida1, Massoud Motamedi2, Ping Wu3
1Departments of Neuroscience, Cell Biology and Anatomy, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX, USA.
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Animal models of traumatic brain injury (TBI) are critical for understanding its complex neuropathology. Here, we present a protocol to induce varying TBI severities in mice using a closed-head, weight-drop model that includes an impact-induced acceleration mechanism. We describe steps for habituation with neurological severity score (NSS) equipment, assessing NSS baseline, performing anesthesia and TBI, assessing NSS post-injury, and analyzing data. This protocol requires no prior surgical intervention and is adaptable for rat studies. For complete details on the use and execution of this protocol, please refer to PhD Dissertation of Javier Allende Labastida1 and Tang et al.2.
