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Protocol to investigate G protein-coupled receptor signaling kinetics and concentration-dependent responses using

Remi Janicot1, Mikel Garcia-Marcos2

  • 1Department of Biochemistry & Cell Biology, Chobanian & Avedisian School of Medicine, Boston University, Boston, MA 02118, USA.

STAR Protocols
|October 12, 2024
PubMed

Keywords:
biophysicsbiotechnology and bioengineeringcell biologycell culturecell-based assaysmolecular biologysignal transduction

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G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
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