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A novel low-cost and simple fabrication technique for a paper-based analytical device using super glue
Hyo-Eun Kang1, The Huy Bui1, Won Han2
1Major of Biomedical Engineering, Division of Smart Healthcare, College of Information Technology and Convergence, Pukyong National University, Republic of Korea.
Analytica Chimica Acta
|October 13, 2024
Summary
This study introduces a simple, low-cost method for fabricating microfluidic paper-based analytical devices (μPADs) using super glue vapor. This innovation makes advanced diagnostics accessible without specialized equipment or training.
Area of Science:
- Materials Science
- Analytical Chemistry
- Biomedical Engineering
Background:
- Microfluidic paper-based analytical devices (μPADs) offer cost-effective, portable solutions for point-of-care testing (POCT) and on-site detection.
- Current μPAD fabrication methods often require specialized equipment and training, limiting their accessibility, especially in resource-limited settings.
- There is a need for simplified, low-cost fabrication techniques for μPADs that do not require specialized expertise or equipment.
Purpose of the Study:
- To develop a novel, efficient, and low-cost method for fabricating μPADs.
- To simplify the fabrication process, making μPADs accessible for use without specialized training or equipment.
- To demonstrate the versatility of the developed method through the fabrication of μPADs capable of multiplexed detection.
Main Methods:
- A new fabrication method utilizing 3D-printed slidable chambers and super glue (ethyl-cyanoacrylate) vapor to create hydrophobic barriers on paper substrates.
- Optimization of exposure sequences to super glue and water vapors, along with controlled heating conditions, for rapid hydrophobization (within 5 minutes).
- Demonstration of selective vapor exposure for creating distinct hydrophilic channels and hydrophobic barriers on paper.
Main Results:
- Achieved rapid (5 min) and effective hydrophobization of paper substrates using ethyl-cyanoacrylate vapor.
- Successfully fabricated μPADs capable of simultaneous detection of multiple analytes, including glucose and proteins.
- Demonstrated simultaneous detection of heavy metal ions (Ni²⁺ and Cu²⁺), showcasing the method's broad applicability in diagnostics.
Conclusions:
- This study presents the first method for selective ethyl-cyanoacrylate vapor exposure in μPAD fabrication.
- The developed technique significantly reduces fabrication complexity, time, and cost, enhancing μPAD accessibility.
- The method is suitable for various settings, requires no specialized equipment, and can be performed by untrained individuals, promoting wider μPAD adoption.
